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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and thus may contribute to tumorigenesis. This protein was observed to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being documented but specific nucleotide sequence information and facts just isn't nonetheless accessible.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally incorporates the epitope acknowledged because of the antibody.
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This guidebook outlines additional doable optimization of cellular and immunoassay parameters to make certain the best possible success are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the target protein. By comparing the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs into the remarkably conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is degraded as cells development via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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